To evaluate the effects of DUB mutants on HD pathology we performed genetic interaction tests by generating flies expressing exon1 of human Huntingtin with a 120 residue long polyQ repeat (Httex1.Q120)16 in the nervous system under the influence of the elav-GAL pan-neuronal driver (HD flies) and simultaneously being heterozygous for DUB mutations, transgenes or RNAi constructs. This evidence concerns the gene HTT and Huntington disease.