To elucidate the detailed mechanism through which HOMER3 and PAFAH1B3 regulate the proliferation and invasion of lung cancer, a TMT-based quantitative proteomics analysis was performed on controls, H1299 cells treated with shHOMER3, and PAFAH1B3-overexpressing H1299 cells treated with shHOMER3, and identified 6, 048 proteins. The gene discussed is PAFAH1B3; the disease is lung cancer.