To examine the potential role of ORMDL3 in metabolically active tissues and the development of NASH, we utilized Ormdl3 overexpressing transgenic mice (ORMDL3TG) fed a Western high-fat diet and sugar water (HFD/SW), previously shown to mimic a ‘western diet’ and promote the development of obesity, hepatic steatosis and dyslipidemia [20]. This evidence concerns the gene ORMDL3 and obesity due to melanocortin 4 receptor deficiency.