The findings of this study regarding the relationship between TMB, C5orf34, and MSI demonstrate that TMB and MSI and C5orf34 expression are positively correlated in a variety of cancer types, including THCA, LUAD, LGG, KIHC, BRCA, SARC, PAAD, STAD, ACC, and BLCA. Here, C5orf34 is linked to cancer.