Figure 3D shows the interference efficiency of the miR-4270 inhibitor. DNMT3A protein and mRNA levels were markedly reduced by miR-4270 upregulation and increased by miR-4270 knockdown (Figs. 3E and 3F). Interestingly, we discovered that DNMT3A levels were substantially elevated in HCC patients compared to controls, shown by Sanger prediction, and were correlated with HCC clinical stage (Figs. 3G and 3H). Additionally, patients with elevated DNMT3A expression had notably poorer disease-free survival than those with low DNMT3A levels (Fig. 3I). The gene discussed is DNMT3A; the disease is hepatocellular carcinoma.