In another study, the expressions of CD8+, CD4+, FOXP3+, Immunoglobulin G4 (IgG4), and IL-10 and clinical information of 118 patients with esophageal squamous cell carcinoma (ESCC) were assessed with hematoxylin and eosin (H&E), immunohistochemistry (IHC) staining and multi-color Immunofluorescence (81). The gene discussed is CD4; the disease is esophageal squamous cell carcinoma.