In another study, the expressions of CD8+, CD4+, FOXP3+, Immunoglobulin G4 (IgG4), and IL-10 and clinical information of 118 patients with esophageal squamous cell carcinoma (ESCC) were assessed with hematoxylin and eosin (H&E), immunohistochemistry (IHC) staining and multi-color Immunofluorescence (81). This evidence concerns the gene IL10 and esophageal squamous cell carcinoma.