To interrogate the spatial intratumoral heterogeneity of HGG, we selected 3 IDH wild-type WHO grade 4 GBM (GBM −1 to 3) and 3 IDH1-mutant grade 3–4 oligodendroglioma (O-1) and astrocytoma (A-2, A-3) samples for spatial whole transcriptome analysis. The gene discussed is IDH1; the disease is astrocytoma (excluding glioblastoma).