To investigate this, we performed qPCR on sorted TCs, FBs, and MØs from the tri-culture tumor spheroids to examine the expression of chemokines (CCL2, CCL7, CCL8, and CCL13) known to induce monocyte chemotaxis through the CCR2 receptor (Fig. 2Ai)34. The gene discussed is CCL8; the disease is neoplasm.