Therefore, in the current study, we investigated the amount of F. nucleatum and its relationship with the expression of inflammatory genes (TLR-2, TLR-4, TNF-α, IL-6, IL-10, IL-12β, and IL-17) and miRNAs (miR-21, and miR-31) in tumor and adjacent normal tissue of Iranian CRC patients. The gene discussed is TLR4; the disease is neoplasm.