Biologicalevaluation of [18F]FDG-Tz in healthy mice indicated favorablepharmacokinetics with quick blood clearance, urinary excretion asthe main elimination route, and the absence of GLUT1 transportation.The successful pretargeted experiments with TCO-functionalized MSNArevealed higher tumor uptake compared to preclicked MSNA in HER2-expressinghuman breast cancer xenograft-bearing mice. This evidence concerns the gene SLC2A1 and breast carcinoma.