Polysaccharide from okra treatment alleviated depressive and anxiety behavior in CUMS mice model, reduced the rising proinflammatory cytokines in the colon, serum, and hippocampus, regulated the gut microbiota profiles and composition, reversed Toll-like receptor 4 (TLR4)/ nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) signaling in hippocampus. Here, TLR4 is linked to Anxiety.