In vitro: Induced G0/G1 cell cycle arrest via decreasing the expression of both cyclinD1/CDK4 and cyclin E/CDK2 in TNBC (70), decreased cell viability and proliferation, colony formation and spheroid growth on metastatic melanoma (69).In vivo: Increased the radiosensitivity of GBM, resulting in increased tumor cell death and prolonged animal survival (71), cytotoxic effects on melanoma brain metastases (69). The gene discussed is CCNE1; the disease is metastatic melanoma.