In these assays, a T-cell stimulator (TCS) cell, which is a murine thymoma-derived cell line (BW5417) engineered to express a membrane-bound single-chain Fv (scFv) variant of the anti-CD3ε antibody OKT3, is used as the APC (Leitner et al, 2010). This evidence concerns the gene CD3E and thymoma.