In this system, the Jurkat reporter cell lines, with or without PD-1, henceforth referred to as Jurkat/PD-1 and Jurkat/control, are stimulated with a previously described TCS, a murine thymoma cell line, BW5417, that co-expresses a membrane-bound single-chain Fv (scFv) variant of the anti-CD3ε antibody OKT3 and high levels of PD-L1 and CD86 (TCS/CD86/PD-L1) (Leitner et al, 2010). The gene discussed is CD274; the disease is thymoma.