A single study reported a decrease in serum ZO-1 from baseline in Thai adults with obesity supplemented with a combined 5 × 1010 CFU/d of Lactobacillus paracasei, Bifidobacterium longum, and Bifidobacterium breve for 12 wk compared to controls [85]. This evidence concerns the gene TJP1 and obesity due to melanocortin 4 receptor deficiency.