CatB, one of the proteins that significantly changes between the A−T−N− and A+T−N−/A+T−N+, is associated with senile plaques in AD brains and is also involved in the lysosomal protease-mediated processing of amyloid precursor protein (APP) to Aβ.25,26 Therefore, we have selected CatB for the validation of proteomics findings in a larger cohort using both CSF and plasma EVs. This evidence concerns the gene APP and Alzheimer disease.