Notably, by analyzing the RNA-seq results of HCC patients from TCGA, we found that high expression of IRG1 in macrophages (CD68highIRG1high) in HCC patients predicts poor prognosis compared with low expression of IRG1 in macrophages (CD68highIRG1low), whereas there is no difference in the survival time between HCC patients with CD68lowIRG1high and CD68lowIRG1low expression (Fig. 1e). Here, ACOD1 is linked to hepatocellular carcinoma.