Conversely, when transfection of GADD45B interfering vector or miR-300 mimics decreased the level of GADD45B in melanoma cells, the number of cells in G1 phase decreased (44.34% and 48.81%) (p-value<0.05), and the cell volume increased in S phase (35.99% and 32.03%) and G2 phase (19.66% and 19.17%) (p-value<0.05), indicating that the proportion of cells undergoing DNA replication and mitosis increased (Figure 3C–3F). The gene discussed is GADD45B; the disease is melanoma.