Utilizing advanced macrolaser light-sheet microscopy in conjunction with state-of-the-art tissue-clearing chemical techniques, we conducted a comprehensive investigation into the three-dimensional spatial distribution of the key neuropathological markers thioflavin S for β-amyloid, anti-CD11b for microglia, and anti-ACSA-2 for astrocytes in the brains of 44-week-old 5xFAD mice, which are Alzheimer’s disease (AD) model mice, and C57BL control mice (Figure 1 and Figure 2, Supplemental Movie S1). Here, ITGAM is linked to early-onset autosomal dominant Alzheimer disease.