TIMP4 and myopia: Our findings linked these variants to high myopia based on the following reasons: (1) the TIMP4 LoF variants are enriched in the high myopia cohort in our study; (2) the TIMP4 LoF variants were predicted to result in truncation changes, disrupting domain with important biological functions; (3) pathological changes resembling those in HM individuals were observed in the Timp4-knockdown rat model in a dose-dependent manner; (4) form deprivation could exacerbate the pathological changes in gene defect rats.