The biochemical analysis revealed significantly elevated levels of serum triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT), aspartate aminotransferase (AST), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), and insulin (INS) in mice with NAFLD compared to the control group, as shown in Figure 1G. These findings indicate the successful establishment of an NAFLD mouse model. Here, GPT is linked to metabolic dysfunction-associated steatotic liver disease.