Taking genetic variance into account, we found that no genetic mutations were detected in tested HNSC cells, whereas only a rare frequency (1.15%) of genetic mutations for the PKM2 gene were in HNSC tissues using the cBioPortal cancer genomic database (http://www.cbioportal.org, accessed on 10 Augest 2023), indicating that genetic variance is not associated with the role of PKM2 in regulating HNSC malignancy (Figure S10). This evidence concerns the gene PKM and cancer.