Although the study on the impact of obesity on Cdc42 activity and expression is limited, there are data demonstrating that after two weeks of a high-fat diet (HFD), C57BL6/J mice exhibited dramatic remodeling of the actin cytoskeleton and increased Cdc42 activity along with increased cell size and impaired insulin signal transduction in adipocytes [214]. Here, INS is linked to obesity due to melanocortin 4 receptor deficiency.