The overexpression of Hint1, specifically in endothelial cells, led to a significant improvement in the recovery of limb ischemia, as demonstrated by an increase in perfusion signal detected by Laser Doppler imaging evaluation (Figure 3B,C) and capillary density (Figure 3D,E) under both basal and STZ-induced hyperglycemic conditions. The gene discussed is HINT1; the disease is limb ischemia.