In addition, compared with healthy patients, the increase in serum levels of free mtDNA in type 2 diabetes mellitus (T2DM) patients is positively correlated with pathology, suggesting that GSDMD may form mitochondrial pores to release mtDNA in cardiomyocytes and that mtDNA plays a crucial role in GSDMD-mediated pyroptosis in T2DM. Here, GSDMD is linked to diabetes mellitus.