Then, we increased the expression of TMEM97 in the MCF7 and T47D cells as they are human breast cancer cell lines with estrogen and progesterone receptors (ER-positive breast cancer cells) by lentivirus infection, with GFP as an indication of successful transductions and predominant localization of TMEM97 in the cytoplasm (Supplemental Figure S1B). The gene discussed is ESR1; the disease is breast carcinoma.