ESR1 and breast cancer: As shown in Figure 1A and Table 2, breast cancer patient tissue samples with the highest ER expression (ER+++) (n = 42, 42%) exhibited the highest H-score of 123.16 ± 8.70, and the H-scores reduced as the ER-positive status declined, with the ER ++ samples (n = 16, 15.7%) scoring 77.93 ± 14.97 and ER + samples (n = 15, 14.7%) scoring 52.50 ± 10.06.