KIT and mastocytosis: To avoid false-negative results in patients suspected of having mastocytosis with mildly elevated serum tryptase levels or subtle symptoms, utilization of highly sensitive methods like digital droplet PCR (ddPCR) and allele-specific oligonucleotide-based PCR (ASO-qPCR) are recommended to screen for KIT D816V mutations in peripheral blood or BM [69,103,104].