Importantly, in several human tumor cells, GLS2 was detected mainly in mitochondria but, in approximately 20% of these cells, it was localized in nuclei; moreover, a significant increase in the amount of GLS2 in nuclei was observed in either SH-SY5Y or HepG2 cells treated with the differentiation agent phorbol 12-myristate 13-acetate (PMA) or T98G glioblastoma cells transfected with the full GLS2 ORF [58]. This evidence concerns the gene GLS2 and neoplasm.