Alongside the heightened expression of immune inhibitory mediators and programmed death ligand 2, the infection of the T. cruzi RA strain triggered an early elevation of Gal-1 expression within living organisms. When compared to the wild-type (WT) mice, Gal-1-deficient (Lgals1−/−) mice demonstrated decreased mortality rates and lower parasite levels in their muscle tissue. This evidence concerns the gene PDCD1LG2 and infection.