BACH1 and infection: To evaluate whether Bach1 plays a role in promoting necrotic cell death in Mtb infection in vitro, we generated bone marrow-derived macrophages (BMDM) from Bach1−/− and WT mice and infected them with H37Rv at a multiplicity of infection (MOI) of 10, since consistent with previous findings, we found no significant difference in macrophage death upon Mtb infection at a low MOI of 1 (Extended Data Fig. 7).