Given the importance of immunotherapy in NSCLC47, it is critical in future studies to combine our approach with functional subtyping of various immunosuppressive immune cells (e.g. CD8 activation, Tregs) and their relation to various EMT states in the tumor microenvironment for a more comprehensive immune profiling of primary tumors, liquid biopsies and metastases. This evidence concerns the gene CD8A and neoplasm.