Excess PrPC (red) was mainly located in the cytoplasm, and abundant miR-214-3p (green dots) and the colocalization of excess PrPC and miR-214-3p (yellow dots in the merged images) were observed not only in the skeletal muscle of six myopathy patients with excessive PrPC (Fig. 1a) but also in differentiating C2C12 cells stably expressing WT PrPC (Fig. 2d). Here, PRNP is linked to myopathy.