Lastly, to determine whether the acute food intake suppressive effects of CAP-GDF15 would lead to long-term suppression of feeding and obesity, we administered CAP-GDF15 or scrambled CAP-GDF15 (50 mg/kg/day, IP), or vehicle control to diet-induced obese mice. Here, GDF15 is linked to obesity due to melanocortin 4 receptor deficiency.