ICD plays an important role in cancer immunotherapy.[6b] To investigate the induction of ICD by DMPtNPS and RT treatment, the ICD biomarkers‐calreticulin (CRT), adenosine triphosphate (ATP), and high mobility group box 1 (HMGB1) were analyzed by immunofluorescence and FCM analysis, respectively. This evidence concerns the gene HMGB1 and cancer.