Next, further to evaluate the clinical relevance of ApoE expression with obesity, OAT samples were collected from 30 overweight/obese (BMI ≥ 24) subjects and 17 lean (BMI < 24) undergoing RNA-Sequencing (RNA-Seq) analysis (Cutoff: P-value < 0.05 and |Log2 (fold change) | > 0.3). This evidence concerns the gene APOE and obesity due to melanocortin 4 receptor deficiency.