POU5F1 can down-regulate the expression of TRIM59, reduce the ubiquitination level of TRAF6, inhibit the ubiquitination degradation of TRAF6, promote the formation of TLR4/TIRAP/TRAF6 complex, promote the activation of NF-κB pathway, and finally promote the proliferation, migration and invasion of GC cells. This evidence concerns the gene TLR4 and gastric cancer.