CBFB and acute myeloid leukemia: These samples also showed predominantly cytoplasmic CBFB::MYH11 aggregates with RUNX colocalization (Figure 5A and Supplemental Figures 2 and 3), and we confirmed that CBFB::MYH11 was primarily cytoplasmic in human AML samples using nuclear-cytoplasmic fractionation and Western blotting.