SREBF1 and steatosis: The increased steatosis was in turn accompanied by the increased expression of de novo lipogenesis genes, including Fasn, Scd1, and Srebf1 (Figure 8E), increased hepatic FASN and SCD1 protein levels (Figure 8F), and an approximately 2-fold increase in de novo lipogenesis (Figure 8G), as determined in liver slices ex vivo, without any significant change in fatty acid oxidation (Figure 8H).