NOX4 and obesity due to melanocortin 4 receptor deficiency: Consistent with this, NFE2L2, NQO1, SOD2, CAT, and NOX4 mRNA levels, as assessed by quantitative real-time PCR (qPCR) (Figure 1B), were also increased in liver core biopsies from patients with obesity (BMI = 36–61) with NAFL (NAS = 1–2), when compared with those from patients with obesity (BMI = 36–61) without steatosis (NAS = 0) (32).