NFE2L2 and steatosis: To test this, we isolated hepatocytes from control (Nox4fl/fl) (45) and hepatocyte-specific NOX4-deficient mice (Alb-Cre Nox4fl/fl) fed a HFD for 10 weeks to induce steatosis (Supplemental Figure 3A) and assessed H2O2 levels and the expression of NFE2L2 and its target genes.