To test this, we isolated hepatocytes from control (Nox4fl/fl) (45) and hepatocyte-specific NOX4-deficient mice (Alb-Cre Nox4fl/fl) fed a HFD for 10 weeks to induce steatosis (Supplemental Figure 3A) and assessed H2O2 levels and the expression of NFE2L2 and its target genes. This evidence concerns the gene NOX4 and steatosis.