NOX4 and metabolic dysfunction-associated steatohepatitis: To independently assess the extent to which NASH and fibrosis may affect Nox4 expression in vivo, we took advantage of a publicly available cell type–resolved transcriptional data set (59) to assess the expression of Nox4, as well as the expression of antioxidant defense genes in hepatocyte nuclei from mice fed a low-fat control diet versus those given a HFD rich in fructose, PA, and cholesterol (HFD-FPC diet) for 20 weeks; the HFD-FPC diet promotes variable degrees of NASH and fibrosis and recapitulates key aspects of human NASH (59).