Notably, the deletion of hepatocyte Nox4 in adult obese mice also facilitated the transition to NASH with fibrosis, as assessed histologically by Picrosirius red staining (Figure 12I) and by measuring hepatic expression of inflammatory (Tnf, Ifng) and fibrosis-related (Acta2, Tgfb, Col1a1, Fn1) genes (Figure 12J) and hepatic hydroxyproline levels (Figure 12K). This evidence concerns the gene COL1A1 and metabolic dysfunction-associated steatohepatitis.