Moreover, tt‐DDE has been attributed with a pivotal role in cardiovascular diseases such as endothelial dysfunction, atherosclerosis, and hypertension, with dysregulation of eNOS being a key feature.[30, 31] The reduction of eNOS also was referred as one of the major cause of vascular complications and induced by insulin resistance in diabetes.[54, 55] Our data revealed that nos2b, an eNOS homolog, was also decreased by tt‐DDE exposure, indicating a modification to the downstream insulin signaling pathway. Here, NOS3 is linked to endothelial dysfunction.