Using these tissues, we have created various tools for breast cancer research including multiple immortalized cell lines with luminal enriched gene expression patterns, a minimum requirement for transformation of these immortalized cell lines, and the generation of a single-cell transcriptome atlas of the breast tissues from women without BRCA1/2 mutations (referred as non-carriers hereafter; refs. 10, 18, 19). This evidence concerns the gene BRCA1 and breast carcinoma.