PPI analysis of 25 TFs revealed that they could be classified into three clusters, with close interaction between green cluster (AR, ARID5A, FOXA1, FOXJ2, FOXL2, FOXN3, GATA2, HNF1A, HNF1B, HOXA13, HOXB13, HSF4, NFIC and PGR) and red cluster (FOXB2, FOXC2, FOXK1, FOXO3, FOXO4, FOXP3, FOXS1 and NFYB), while blue cluster (HOXC10, HOXC12 and HOXC13) was independent, suggesting that blue cluster may have a unique regulatory pattern in PCa progression (Fig. 1B). This evidence concerns the gene FOXO4 and posterior cortical atrophy.