Based on the peripheral blood RNA sequencing data of the normal control group (n=10) and the sepsis group (n=22), the expression of core genes was plotted pod plots, and the results showed that GNLY, GZMB, PRF1 and RASGRP1 were highly expressed in the normal control group and low in the sepsis group, and the difference was statistically significant (Fig. 7). The gene discussed is PRF1; the disease is Sepsis.