In addition, treatment of xenografted HuCCT1 cells with low-dose volasertib (10 mg/kg) repressed tumor growth, and a combination of low-dose volasertib and low-dose GEM (10 mg/kg) treatment further decreased tumor growth (Fig. 6D), supporting the role of PLK1 and MISP in regulating cell proliferation in vitro and tumorigenesis in vivo in iCCA cells. Here, MISP is linked to neoplasm.