To verify that miR-29c-3p/SERPINH1 axis regulated the Wnt signaling pathway in ESCC cells, we examined the expression of Wnt pathway-related proteins by Western blot, which manifested that inhibition with SERPINH1 remarkably suppressed levels of Wnt-1 and β-catenin protein, while increasing p-β-catenin and AAK1 protein levels over the NC-inhibitor+sh-NC group. This evidence concerns the gene SERPINH1 and esophageal squamous cell carcinoma.