Subsequent survival analysis of these hub genes was performed using cBioPortal, revealing that ACC patients with alterations in CDK1, CCNA2, CCNB1, BIRC5, BUB1, and AURKA (depicted by the red solid line) demonstrated significantly lower overall survival rates and disease free survival rates compared to patients without alterations in these genes (depicted by the blue solid line). The gene discussed is BUB1; the disease is adrenal cortex carcinoma.