In this paper, we used the TCGA database and GEO database to analyze the pan-cancer analysis of ASS1, including the expression of the ASS1 gene and protein in different tumors, the impact of ASS1 on tumor survival, changes in immune-infiltrating cells and related cellular pathways, etc., to explore the potential molecular mechanism of ASS1 in the pathogenesis or clinical outcomes of different tumors. Here, ASS1 is linked to neoplasm.