At the end of the study, the average volume size of a tumor for mice treated with the AdV5/3-D24, Ad5/3-D24-ICOSL-CD40L, and in their combinatory therapy was, respectively, 56, 55, and 56, 50 mm3 compared to 99 mm3 (anti-PD-1) and 95 mm3 (control). The gene discussed is PDCD1; the disease is neoplasm.