To understand the functional capacity of sCTLA-4 to modulate anti-tumor T cell responses, we constructed expression vectors to generate tumor cells that constitutively secrete recombinant sCTLA-4 and created stable cell lines (Figures 1B and 1C) but which, importantly, do not express surface CTLA-4 (Figure S2). The gene discussed is CTLA4; the disease is neoplasm.