As shown in Fig. 4A–E, we found that the expression level of all the selected hub genes was more significantly decreased in AD+ mice than in AD− mice (SNAP25, 0.74 ± 0.15, P = 0.0005; SYN1, 0.70 ± 0.23, P = 0.0037; SYT1, 0.67 ± 0.30, P = 0.0129; GAP43, 0.68 ± 0.27, P = 0.0079; SNAP91, 0.80 ± 0.16, P = 0.0064). This evidence concerns the gene SYN1 and Alzheimer disease.