Overall, TMB and TIMELESS expression were positively associated in fifteen different cancer types., including ACC, LUAD, LUSC, PRAD, UCEC, BLCA, SARC, BRCA, MESO, COAD, STAD, SKCM, KIRC, HNSC and LGG (Additional file 3: Table S2; Fig. 7a), and negatively correlated with TMB in ESCA and THCA, (Additional file 3: Table S2; Fig. 7a). Here, TIMELESS is linked to cancer.