Next, different HCC cell lines were used to evaluate how the activity of STAT3 was regulated and we found that both S-palmitoylation (Fig. 1G) and phosphorylation (Fig. 1, G and H) of STAT3 could be induced by DHHC7 WT while APT2 inhibition with ML349 (a specific APT2 inhibitor)(1) only caused minor perturbation to STAT3 phosphorylation (Fig. 1G). The gene discussed is STAT3; the disease is hepatocellular carcinoma.